Play-off chasing Rochdale were humbled on the road by Conference side Forest Green Rovers. Smith gave Forest Green a 27th minute lead, with Low doubling the advantage in the 56th minute to put the Conference side in to the Third round for the first time in their history.
Brentford also slipped up against non-League opposition. Conference side Barrow knocked the League Two promotion hopefuls out with a 2-1 win against a 10-man Bees side. Hamer was sent off for Brentford in the 36th minute. Three minutes later Brown netted from the penalty spot. MacDonald levelled for the Bees in the 50th minute only to see Henney score the winner in the 71st minute.
AFC Bournemouth were held at Dean Court by first round giant killers Blyth Spartans of the Conference North in a 0-0 draw. The Spartans keeper twice denied Lee Bradbury with some acrobatic saves to force a replay.
Gillingham came closest to knocking out a League One side by holding Stockport County to a 0-0 draw at Priestfield.
Bradford City, 2nd in League Two went out to Leyton Orient at Valley Parade. Demetriou gave the east London side a 14th minute lead. Boulding levelled in the 58th minute only to see Granville score the winner in the 65th minute.
Dagenham & Redbridge travelled to high flying Leicester City and gave a good account of themselves, but eventually went down 3-2 at The Walkers Stadium. Ritchie stunned the home crowd with a 9th minute goal. City struck back 2 minutes later through Fryatt. Strevens gave the Daggers a 2-1 lead in the 25th minute, with Fryatt levelling for the second time from the penalty spot. Fryatt completed his hat trick in the 55th minute.
Aldershot Town went down 3-0 at League One Millwall at The New Den. A brace from Alexander in the 30th & 77th minutes set the win up with Grimes putting the result beyond doubt in the 88th minute.
Southend United dumped Luton Town out of the FA Cup with a 3-1 win at Roots Hall. Stanislas netted a brace in the 34th and 84th minutes, either side of a goal from Spillane in the 80th minute for the Hatters. The result was put beyond doubt when Walker netted in the 90th minute.
Port Vale and Macclesfield played out the only all-League Two encounter at Vale Park. Local bragging rights going to the Silk Men after a 3-1 win. Green gave the visitors a 9th minute lead, which stuck until Dodds levelled in the 71st minute. Green restored the Silk Men's advantage in the 73rd minute with Gritton putting the result beyond doubt in the 90th minute.
Chesterfield and giant killers Droylsdon will have to try again after their match was abandoned die to fog. This after Lamb had given the non-leaguers a 35th minute lead.
Morcambe and Cheltenham Town will have to try again as their match was abandoned in the 64th minute due to fog with the scores level at 1-1. Howe had given Morcambe a 15th minute lead with Hayles levelling in the 54th minute.
Notts County are the remaining League Two to play this weekend as they take on Conference North side Kettering at Meadow Lane.
In League Two, Accrington Stanley hosted local rival Bury at Livingstone Road. Two quick strikes by Bury through Jones (35th min) and Barry-Murphy (38th min) only drew a response from Clarke (79th min penalty) to give 3-points to the visitors who climb back in to the play-off places. Accrington Stanley 16th, Bury 6th.